Page 8 - Instruction Manual 2

Pre-Rinse the Tips
some liquids (e.g., protein-containing solutions
and organic solvents) can leave a film of liquid
on the inside wall of the tip; pre-rinse the tip to
minimize any errors that may be related to this
phenomenon.
PIPETTING Pre-rinsing consists of aspirating the first
volume of liquid and then dispensing it back to
waste. subsequent volumes that you pipette
will have levels of accuracy and precision within
specifications.

Aspirate
1. Press the push button to the first stop (this
corresponds to the set volume of liquid).
2. Hold the pipette vertically and
immerse the tip in the liquid (refer to
Guidelines for Good Pipetting on page 9).
Release the push button slowly and smoothly
(to top position) to aspirate the set volume of
liquid. Wait one second, the time depends on
the model (refer to the table), then withdraw
the pipette tip from the liquid. You may wipe
any droplets away from the outside of the tip
using a medical wipe; however, if you do so,
take care to avoid touching the tip’s orifice.

First stop
Second stop

Figure 6
Pipetting Motion - Aspirate and Dispense

8 PIPETTING | PIPETMAN® ClAssIC PIPETMAN® ClAssIC | UsER’s GUIDE