Page 9 - Instruction Manual 2

Dispense
1. Place the end of the tip against the inside
wall of the recipient vessel (at an angle of 10°
to 40°).
2. Press the push button slowly and smoothly
to the first stop.
3. Wait for at least a second, then press the
push button to the second stop to expel PIPETTING
any residual liquid from the tip. Keep the
push button pressed fully down and (while
removing the pipette) draw the tip along the
inside surface of the vessel.
4. Release the push button smoothly. Eject the
tip by pressing firmly on the tip of the ejector
button.
Guidelines for Good Pipetting

IMMERsION DEPTH WAIT TIME
MODEl
(MIllIMETERs) (sECONDs)
P2 1 1
P10 1 1
P20 2–3 1
P100 2–4 1
P200 2–4 1
P1000 2–4 2–3
P5000 3–6 4–5
P10mL 5–7 4–5
1. Make sure that you operate the push button
slowly and smoothly.
2. When aspirating, keep the tip at a constant
depth below the surface of the liquid (refer
to the table).
3. Change the tip before aspirating a different
liquid, sample, or reagent.
4. Change the tip if a droplet remains at the
end of the tip from the previous pipetting
operation.
5. Each new tip should be pre-rinsed with the
liquid to be pipetted.

PIPETTING | PIPETMAN® ClAssIC PIPETMAN® ClAssIC | UsER’s GUIDE 9